《相关文摘》：毛细管电泳-电化学检测法测定蜘蛛香中多元酚类化合物

【英文篇名】Determination of Polyphenolic Constituents in Valeriana Jatamansi Jones by Capillary Electrophoresis with Electrochemical Detection【作者】傅亮; 楚清脆; 黄宝康; 郑汉臣; 叶建农;【英文作者】Fu Liang 1; Chu Qingcui 1; Huang Baokang 2; Zheng Hanchen 2; Ye Jiannong *1 1（Department of Chemistry; East China Normal University; Shanghai 200062）2（School of Pharmacy; Second Military Medical University; Shanghai 200433）;【单位】华东师范大学化学系; 第二军医大学药学院;【刊名】分析化学 , 编辑部邮箱 2005年02期 中文核心期刊要目总览ASPT来源刊中国期刊方阵CJFD收录刊【英文刊名】Chinese Journal of Analytical Chemistry【中文关键词】毛细管电泳; 电化学检测; 蜘蛛香; 多元酚;【英文关键词】Capillary electrophoresis; electrochemical detection; Valeriana jatamansi Jones; polyphenols;【中文摘要】采用毛细管电泳 电化学检测法(CE ED)同时测定了蜘蛛香根中香叶木素、山奈酚、芹菜素、绿原酸 和咖啡酸等5种主要生物活性成分的含量,考察了运行缓冲液酸度、浓度、分离电压、氧化电位和进样时间等 实验参数对分离、检测的影响。在最佳实验条件下,以直径300μm的碳圆盘电极为工作电极,检测电位为+ 950mV(vs.SCE),在50mmol/L的硼砂缓冲溶液(pH9.23)中,上述各组分在23min内能完全分离。5种组 分在两个数量级的范围内呈良好线性关系,检测下限(S/N=3)达1.7×10-4～1.8×10-5g/mL。该法已成功 地应用于蜘蛛香根中活性成分的分离检测,结果令人满意。【英文摘要】A high-performance capillary electrophoresis with electrochemical detection ( CE-ED) method has been developed for the determintion of five bioactive ingredients in Valeriana jatamansi Jones, namely diosmetin, kaempferol, apigenin, chlorogenic acid, and caffeic acid. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time on CE-ED were investigated. Under the optimum conditions, these five components can...【DOI】CNKI:ISSN:0253-3820.0.2005-02-003